ISBN 9783843916875

978-3-8439-1687-5, Reihe Organische Chemie

Roman Kolb
β-Sultams as Tools for the Study of Enzyme Function

313 Seiten, Dissertation Technische Universität München (2014), Softcover, A5

Zusammenfassung / Abstract

A diverse set of beta-sultams, the sulfur analogs of beta-lactams, was synthesized and tested for their protein reactivity. These alkynylated compounds were therefore incubated with different bacterial strains and their target profile was analyzed via SDS-PAGE. Target analysis via HR-MS revealed an azoreductase in B. thailandensis and L. monocytogenes, which was verified by overexpression and activity studies. Additionally the inhibition of ClpP by beta-Sultams was investigated and the selective conversion of its active site serine into dehydroalanine was revealed.