ISBN 9783843930246

978-3-8439-3024-6, Reihe Biotechnologie

Lin Chen
Rational Metabolic Engineering and Systematic Analysis of Escherichia coli for L-Tryptophan Bioproduction

187 Seiten, Dissertation Technische Universität Hamburg-Harburg (2016), Softcover, A5

Zusammenfassung / Abstract

In this work, an E. coli strain capable of efficiently producing L-trp has been rationally designed and constructed by targeted genetic modifications on the chromosome. The strain design strategies include (1) inactivation of the tryptophan degradation pathway by deleting the gene tnaA, (2) interruption of the L-trp uptake system by knocking out the genes tnaB and mtr, (3) deregulation of the common aromatic amino acid biosynthesis pathway by removing the feedback inhibition and repression of the 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase, (4) deregulation of the L-serine biosynthesis pathway by introducing a serine-resistant mutant of D-3-phosphoglycerate dehydrogenase, and (5) deregulation of the L-trp branch pathway by eliminating the feedback inhibition, repression, and attenuation of the wild-type trp operon.