ISBN 9783843930741

978-3-8439-3074-1, Reihe Biologie

Thomas Smylla
Mechanisms of Repression of Notch Signaling in Drosophila melanogaster

165 Seiten, Dissertation Universität Hohenheim Stuttgart-Hohenheim (2016), Softcover, A5

Zusammenfassung / Abstract

The Notch signaling pathway is a versatile mechanism of intercellular communication which is broadly utilized in metazoans to convey spatial information and genetic instructions crucial for correct development. Thusly, dysregulation has been linked to a multitude of congenital diseases and cancers in various animals, including humans. Exposing the underlying mechanisms of this signaling pathway on a molecular level is considered vital for the future development of therapeutic approaches.

This work focused on a certain set of aspects of the Notch antagonist Hairless (H) in Drosophila and highlighted their individual significance in a developmental context. These aspects were mainly (1) translational control via internal ribosome entry, (2) isoform availability, (3) binding to Suppressor of Hairless [Su(H)], (4) subcellular localization, and (5) nucleocytoplasmic shuttling. Misexpression experiments and genome engineering approaches were conducted to assess their respective impact on imaginal development of D. melanogaster.