ISBN 9783843946322

978-3-8439-4632-2, Reihe Thermodynamik

Thorsten Greinert
Thermodynamics of Glycolysis

190 Seiten, Dissertation Technische Universität Dortmund (2020), Softcover, A5

Zusammenfassung / Abstract

Thermodynamic feasibility analyses help evaluating the feasibility of metabolic pathways, which is an important information used to develop new biotechnological processes and to understand metabolic processes in cells. However, literature standard data are uncertain for most biochemical reactions yielding wrong statements concerning their feasibility.

In this work, thermodynamics of glycolytic reactions triosephosphate isomerase, glyceraldehyde 3-phosphate dehydrogenase and enolase were investigated. The experimental investigation of the influence of substrate concentrations as well as crowders and salts on the reaction equilibria showed that they depend strongly on these reaction conditions. These influences were accounted for by means of activity coefficients, determined with the equation of state ePC-SAFT in order to gain new standard Gibbs energy of reaction values for the three experimentally investigated reactions as well as for the glycolytic reactions phosphofructokinase, aldolase and phosphoglycerate mutase.

As a result, activity-based standard values for all glycolytic reactions are available and a feasibility analysis was performed. This showed that salts as well as the concentrations of all metabolites within the pathway have a significant influence on the feasibility of the single glycolytic reactions. It was possible to correctly determine the feasibility of the glycolytic pathway.