ISBN 9783843955225

978-3-8439-5522-5, Reihe Mikrosystemtechnik

Atefeh Akbari
Alginate-based Hydrogels for Controlled Delivery of the AGR2 Protein in Tissue Engineering

174 Seiten, Dissertation Albert-Ludwigs-Universität Freiburg im Breisgau (2024), Softcover, B5

Zusammenfassung / Abstract

In this work, these challenges are addressed by synthesizing a novel hybrid system consisting of beads composed of sodium alginate (SA) and carboxymethyl cellulose (CMC). The SA-CMC beads are produced by an ionic gelation technique and loaded with bioactive molecules, in this case anterior gradient 2 (AGR2), bovine serum albumin (BSA) and interleukin-6 (IL-6), as target molecules. In order to better control the dissolution of the protein-loaded alginate beads, they are embedded in films of SA and polyvinyl alcohol (PVA), which are produced using a simple ion gelation technique. This provides materials for tissue engineering with spatially and temporally controlled release of the loaded proteins. The protein release kinetics and accumulation in the hybrid system was investigated by varying the concentrations of the polymers composing the beads and the cross-linking density of the hybrid system. The effects of different concentrations of structural components and the addition of viscosity modifiers on the encapsulation efficiency and the in vitro protein release profiles were investigated. The results show that high concentrations (2 %) of CMC in the SA beads significantly increase the encapsulation efficiency of AGR2, BSA and IL-6, emphasizing the importance of viscosity modifiers and IPN formation for protein accumulation.