ISBN 978-3-8439-4867-8

978-3-8439-4867-8, Reihe Lebensmittelchemie

Qiong Wu
Novel approaches including countercurrent chromatography (CCC) and gas chromatography with stable isotope ratio mass spectrometry (GC-IRMS) in the analysis of halogenated natural products (HNPs) in marine food from different origins

227 Seiten, Dissertation Universität Hohenheim Stuttgart-Hohenheim (2021), Softcover, A5

Zusammenfassung / Abstract

Halogenated natural products (HNPs) are a structurally diverse class of usually polychlorinated and/or polybrominated compounds mainly produced in the marine environment. Investigation during the past two decades produced evidence that some HNPs are similar to hazardous anthropogenic persistent organic pollutants (POPs) in terms of structure and environmental properties, that is, they were also found to be lipophilic, persistent and bioaccumulative. These POP-like HNPs have occasionally been detected in environmental samples and marine food. In consequence they may pose a risk to the human body and some have already been classified as emerging contaminants. Compared with POPs the occurrence and distribution of HNPs in the environment is difficult to predict due to the uncertainty of their natural producers, and little is known about the ways they enter the environment. Another obstacle to the in-depth study of HNPs (including their toxicity) is the lack of reference standards. Some HNPs such as MHC-1 have complex structures, which makes it difficult to synthesise them in the laboratory.

The objective of this thesis was to use GC/MS for the quantitative and qualitative analysis of HNPs especially in marine food. The species included chokka squid (Loligo reynaudii) and sardine (Sardinops sagax) from sites in both the Atlantic and the Indian Oceans off of South Africa, and large pelagic fish from the Seychelles and further areas in the Western Indian Ocean. The quantitative data were used to conduct a risk assessment of HNPs exposure caused by consumption of these marine foods. More information on the potential enrichment of MHC-1 in its producer Plocamium cartilagineum from Heligoland, Germany, and other species in the same habitat was obtained by stable isotope ratio mass spectrometry (IRMS). The potential of countercurrent chromatography (CCC) in the isolation of HNP was explored by the separation of MHC-1 from P. cartilagineum.